dc.contributor.advisor | Tušerová, Jindra | |
dc.contributor.author | Pospíšilová, Kristýna | |
dc.date.accessioned | 2022-03-08T14:21:33Z | |
dc.date.available | 2022-03-08T14:21:33Z | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-04-17 | |
dc.identifier.uri | https://dspace.jcu.cz/handle/123456789/38481 | |
dc.format | 30 | |
dc.format | 30 | |
dc.language.iso | eng | |
dc.publisher | Jihočeská univerzita | cze |
dc.rights | Bez omezení | |
dc.subject | Lepidoptera | eng |
dc.subject | Leptidea wood white butterflies | eng |
dc.subject | Multiple sex chromosomes | eng |
dc.subject | Fluorescence in situ hybridization | eng |
dc.subject | Chromosomal rearrangements | eng |
dc.subject | Speciation | eng |
dc.title | Analysis of structure and origin of multiple sex chromosomes in <i>Leptidea</i> wood white butterflies | cze |
dc.title.alternative | Analysis of structure and origin of multiple sex chromosomes in <i>Leptidea</i> wood white butterflies | eng |
dc.type | bakalářská práce | cze |
dc.identifier.stag | 48971 | |
dc.description.abstract-translated | Previous studies have shown a dynamic karyotype evolution and the presence of complex sex chromosome systems with 3-4 W chromosomes and 3-6 Z chromosomes in Leptidea wood white butterflies. To dissect the evolutionary history of multiple Z chromosomes of Leptidea species, we used identified and selected bacterial artificial chromosome (BAC) clones containing orthologous genes of Bombyx mori chromosome Z and 17, isolated them from the available BAC library and used them as probes for physical mapping by BAC-FISH, first in L. juvernica, then in the closely related L. sinapis. In both Leptidea species, the majority of BAC clones corresponding to the linkage group Z of the B. mori reference genome hybridized to one chromosome of the complicated sex chromosome multivalent. Thus, we named it as Z1 chromosome. Location of all Z-derived BAC clones was identical in both species suggesting a conserved synteny and gene order between L. juvernica and L. sinapis Z1 chromosome. Moreover, our findings indicate that the Z1 chromosome is probably the ancestral Z chromosome in the genus Leptidea. Results of BAC-FISH mapping with clones corresponding to the linkage group 17 of the B. mori reference genome revealed the fusion/translocation event between an ancestral Z chromosome and the chromosome corresponding to B. mori chromosome 17 and supported a previous hypothesis about the role of chromosomal rearrangements in the formation of multiple sex chromosomes in Leptidea butterflies. | eng |
dc.date.accepted | 2018-05-23 | |
dc.description.department | Přírodovědecká fakulta | cze |
dc.thesis.degree-discipline | Biologie | cze |
dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
dc.thesis.degree-name | Bc. | |
dc.thesis.degree-program | Biologie | cze |
dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
dc.contributor.referee | Majtánová, Zuzana | |