| dc.contributor.advisor | Chmelař, Jindřich | |
| dc.contributor.author | Chlastáková, Adéla | |
| dc.date.accessioned | 2021-12-08T12:56:31Z | |
| dc.date.available | 2021-12-08T12:56:31Z | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2016-12-14 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/123456789/33992 | |
| dc.format | 81 s. | |
| dc.format | 81 s. | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Ixodes ricinus | eng |
| dc.subject | cystatins | eng |
| dc.subject | neutrophils | eng |
| dc.subject | monocytes | eng |
| dc.subject | eosinophils | eng |
| dc.subject | thioglycollate-induced peritonitis | eng |
| dc.subject | carrageenan-induced paw edema | eng |
| dc.title | Zavedení a optimalizace <i>in vivo</i> modelů zánětu a jejich využití pro funkční analýzu inhibitorů proteáz z klíštěcích slin | cze |
| dc.title.alternative | Establishment and optimization of <i>in vivo</i> models of inflammation and their use for functional analysis of tick salivary protease inhibitors | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 41585 | |
| dc.description.abstract-translated | Two murine models of acute inflammation, namely thioglycollate-induced peritonitis and carrageenan-induced paw edema, were optimized using non-steroidal anti-inflammatory drug indomethacin and corticosteroid dexamethasone. During the optimization phase, the presence of neutrophils, monocytes, macrophages, eosinophils, B cells and T cells in the peritoneal cavity at various time points after injection of thioglycollate medium was assessed via multicolor flow cytometry. Moreover, two different thioglycollate media (suppliers BD and Sigma-Aldrich) were compared for their ability to induce an inflammatory response. The optimization of thioglycollate-induced peritonitis and carrageenan-induced paw edema was followed by the evaluation of the anti-inflammatory activity of Ixodes ricinus cystatins G1 and G9 in both mouse models. | eng |
| dc.date.accepted | 2017-01-17 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Klinická biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Kopecký, Jan | |
| dc.contributor.referee | Neuwirth, Aleš | |
