| dc.contributor.advisor | Kopáček, Petr | |
| dc.contributor.author | Kučera, Matěj | |
| dc.date.accessioned | 2021-12-06T14:19:16Z | |
| dc.date.available | 2021-12-06T14:19:16Z | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-04-23 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/123456789/24770 | |
| dc.description.abstract | Tato diplomová práce je zaměřená na posouzení vlivu složek diety na mikrobiální proliferaci ve střevech klíštěte Ixodes ricinus. Byla posouzena dynamika patogenu Borrelie afzelii v závislosti na přítomnosti/nepřítomnosti hemoglobinu v potravě. Byl demonstrován vliv střevního enzymu, duální oxidázy, na regulaci střevní bakteriální mikroflóry. Na základě výsledků RNA interference byl rovněž naznačen vliv duální oxidázy na ovipozici I. ricinus. Dodatek této práce popisuje optimalizaci klíštěcího in vitro krmeni pro nymfy I. ricinus. | cze |
| dc.format | 52 | |
| dc.format | 52 | |
| dc.language.iso | cze | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | Ixodes ricinus | cze |
| dc.subject | in vitro krmeni | cze |
| dc.subject | hemoglobin | cze |
| dc.subject | hem | cze |
| dc.subject | komplement | cze |
| dc.subject | NADPH oxidáza | cze |
| dc.subject | DUOX | cze |
| dc.subject | duální oxidáza | cze |
| dc.subject | Ixodes ricinus | eng |
| dc.subject | in vitro feeding | eng |
| dc.subject | hemoglobin | eng |
| dc.subject | heme | eng |
| dc.subject | complement | eng |
| dc.subject | NADPH oxidase | eng |
| dc.subject | DUOX | eng |
| dc.subject | dual oxidase | eng |
| dc.title | Influence of dietary components and redox enzymes on intestinal microbiota proliferation in the tick <i>Ixodes ricinus</i> | cze |
| dc.title.alternative | Influence of dietary components and redox enzymes on intestinal microbiota proliferation in the tick <i>Ixodes ricinus</i> | eng |
| dc.type | diplomová práce | cze |
| dc.identifier.stag | 35031 | |
| dc.description.abstract-translated | In this work, we have analysed the temporal dynamics of gut-dwelling bacteria and Borrelia in the gut of the deer tick Ixodes ricinus. Using quantitative PCR, we have shown that levels of the tick intestinal microflora are profoundly decreased at later stages of feeding on whole blood but not on serum. Even though we noted that host complement system manages to interfere with Borrelia viability in vitro, we did not see any effect of host complement on Borrelia acquisition in adult ticks in vivo. However, we revealed that host hemoglobin is essential for Borrelia proliferation in the tick gut. All together, these data imply that, during feeding, levels of gut-dwelling bacteria and Borrelia are determined by the host. While hemoglobin seems to be detrimental for gut-dwelling bacteria, Borrelia require it in order to proliferate. During off-host stage, we showed that levels of gut-dwelling microflora are regulated by an intestinal transmembrane enzyme Dual oxidase. In conclusion, we aimed, and mostly succeeded, to perform pilot experiments describing the biology of a complex process of regulating gut microflora in the vector Ixodes ricinus and extend it by its impact on Borrelia acquisition | eng |
| dc.date.accepted | 2015-05-27 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Experimentální biologie - specializace Buněčná a vývojová biologie | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Mgr. | |
| dc.thesis.degree-program | Biologie | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Horáková, Eva | |
| dc.contributor.referee | Chmelař, Jindřich | |
