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dc.contributor.authorŠimůnková, Petra
dc.date.accessioned2021-12-06T14:06:56Z
dc.date.available2021-12-06T14:06:56Z
dc.date.issued2014
dc.date.submitted2014-02-10
dc.identifier.urihttps://dspace.jcu.cz/handle/123456789/24587
dc.language.isoeng
dc.publisherJihočeská univerzitacze
dc.rightsBez omezení
dc.titleCell cycle arrest as a hallmark of insect diapause: Changes in gene transcription during diapause induction in the drosophilid fly, Chymomyza costatacze
dc.typerigorózní prácecze
dc.identifier.stag36574
dc.description.abstract-translatedThe changes of relative mRNA levels of seven different genes, coding for key cell cycle regulatory factors (Cyclins D and E, kinases Wee1 and Myt1, Phosphatase Cdc25 (String), Dacapo (p27), and Pcna) were performed using qRT-PCR method. Two reference genes (Rp49 and ?-tubulin) served as a background. Significant transcriptional response to photoperiodic transfer were observed for two genes. While the relative levels of Dacapo mRNA increased during the rapid entry into G2 arrest, the Pcna expression was significantly downregulated during the beginning of G0/G1 arrest. Moderate transcriptional upregulations of the genes coding for two cell cycle inhibitory kinases, Wee1 and Myt1 accompanied the entry into diapause. The other genes were expressed equally in all photoperiodic conditions.eng
dc.date.accepted2014-02-26
dc.description.departmentPřírodovědecká fakultacze
dc.thesis.degree-disciplineExperimentální biologiecze
dc.thesis.degree-grantorJihočeská univerzita. Přírodovědecká fakultacze
dc.thesis.degree-nameRNDr.
dc.thesis.degree-programBiologiecze
dc.description.gradeDokončená práce s úspěšnou obhajoboucze


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