| dc.contributor.advisor | Grunclová, Lenka | |
| dc.contributor.author | Absolonová, Markéta | |
| dc.date.accessioned | 2021-12-06T13:40:22Z | |
| dc.date.available | 2021-12-06T13:40:22Z | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-05-20 | |
| dc.identifier.uri | https://dspace.jcu.cz/handle/123456789/23783 | |
| dc.format | 22 | |
| dc.format | 22 | |
| dc.language.iso | eng | |
| dc.publisher | Jihočeská univerzita | cze |
| dc.rights | Bez omezení | |
| dc.subject | IrAM4 | eng |
| dc.subject | ?2-macroglobulin | eng |
| dc.subject | Ixodes ricinus | eng |
| dc.subject | protease inhibitor | eng |
| dc.title | IrAM4: Partial characterisation of a molecule similar to \recke{alpha}<sub>2</sub>-macroglobulin from a tick <i>Ixodes ricinus</i> | cze |
| dc.title.alternative | IrAM4: Partial characterisation of a molecule similar to \recke{alpha}<sub>2</sub>-macroglobulin from a tick <i>Ixodes ricinus</i> | eng |
| dc.type | bakalářská práce | cze |
| dc.identifier.stag | 19686 | |
| dc.description.abstract-translated | Ixodes ricinus is a hard tick that can transmit several diseases that are capable of affecting
humans. Among those are tick-borne encephalitis and Lyme disease. The focus of this work is on
IrAM4, a member of tick ?2-macroglobulin family (?2M-F) of proteins which belong to the
evolutionarily oldest constituents of the innate immune system. ?2-macroglobulins are protease
inhibitors and act primarily in inactivation of proteases secreted by invading pathogens within
their infection cycle. The aim of this study was to identify ?2-M of Ixodes ricinus (IrAM4) from
corresponding ortholog of protein ?2-M named IsAM4 present in the genome of closely related
Ixodes scapularis. The partial sequence was determined by amplification of cDNA and
subsequent sequencing of PCR products. RT-PCR tissue profiling revealed that IrAM4 is present
in ovaries and salivary glands but not in the tick gut. The recombinant fragment of IrAM4 was
afterwards prepared for immunization of a rabbit and the obtained polyclonal antibodies were
used for Western blot analysis. The results showed that IrAM4 is mainly present in the
hemolymph and probably in salivary glands and ovaries but it is not expressed in the gut. The
native IrAM4 seems to be composed of two disulfide bound subunits. However, the exact
structure of the molecule was not analyzed in this work. | eng |
| dc.date.accepted | 2011-06-20 | |
| dc.description.department | Přírodovědecká fakulta | cze |
| dc.thesis.degree-discipline | Biological Chemistry | cze |
| dc.thesis.degree-grantor | Jihočeská univerzita. Přírodovědecká fakulta | cze |
| dc.thesis.degree-name | Bc. | |
| dc.thesis.degree-program | Biochemistry | cze |
| dc.description.grade | Dokončená práce s úspěšnou obhajobou | cze |
| dc.contributor.referee | Urbanová, Veronika | |
